TRIzol Reagent

RNA isolation / purification Tissue - Human Seminal vesicles

Experiment
RNA isolation / purification Tissue - Human Seminal vesicles
Product
TRIzol Reagent from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Protocol tips
- For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it.

Publication protocol

To verify the specificity of VASA IC in germ cells, bladder and seminal vesicle epithelium high quality total RNA was extracted from normal testis-parenchyma (n = 10), semen of men with normospermia (n = 8), NOA (n = 5), OA (n = 6), bladder (n = 3), and seminal vesicles (n = 10) using TRIzol Reagent (Life Technologies, Bleiswijk, the Netherlands) according to the manufacturer's instructions. qRT-PCR was performed in duplicate using mRNA probes VASA hs00987125_m1 and endogenous control hypoxanthine-guanine phosphoribosyltransferase (HPRT)1 (4333768F; Life Technologies). Relative VASA transcript abundance was calculated using the 2-dCt method (VASA mRNA =   mean Ct HPRT – mean Ct VASA)

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Discussion

Discussion

4 years ago

Author: Paul G. Macon United States

RNA isolation from tissue

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?

Discussion

5 years ago

Author: Aaron Stege Netherlands

Problem in phase separation after using serum/plasma kit

I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Tissue - Human Seminal vesicles using TRIzol Reagent from Thermo Fisher Scientific.

Paper title
VASA mRNA (DDX4) detection is more specific than immunohistochemistry using poly- or monoclonal antibodies for germ cells in the male urogenital tract
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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for TRIzol Reagent below.

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Videos

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